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example 1  (Sino Biological)


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    Structured Review

    Sino Biological example 1
    Example 1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b7+h3+protein/Human+B7-H3+%2F+CD276+Protein/us12606625-259-8-19
    Average 94 stars, based on 16 article reviews
    example 1 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: EBV-Upregulated B7-H3 Inhibits NK cell–Mediated Antitumor Function and Contributes to Nasopharyngeal Carcinoma Progression
    Article Snippet: The following antibodies were used for IHC staining in this study: B7-H3 (Cell Signaling Technology, catalog no. 14058, RRID: AB_2750877, 1:400) and PD-L1 (Roche, catalog no. 790-4905, RRID: AB_2819099), and horseradish peroxidase (HRP)-conjugated goat anti-mouse/rabbit IgG secondary antibodies (Dako, K5007). .. The following antibodies and other reagents were used for flow cytometric analysis: anti-human FcR block (BioLegend, catalog no. 422302, RRID:AB_2818986), LiveDead-NIR (catalog no. L34975, Thermo Fisher Scientific), anti–CD19-APC-Cy7 (BioLegend, catalog no. 302218, RRID: AB_314248), anti–CD14-APC-Cy7 (BioLegend, catalog no. 367112, RRID:AB_2566714), anti–CD3-FITC (BioLegend, catalog no. 300406, RRID:AB_314060), anti–CD3-Brilliant Violet 650 (BioLegend, catalog no. 317324, RRID:AB_2563352), anti–CD3-Alexa Fluor 700 (BioLegend, catalog no. 317340, RRID: AB_2563408), anti–CD56-APC (BioLegend, catalog no. 304610, RRID:AB_314452), anti–CD56-PE-Cy7 (BioLegend, catalog no. 304628, RRID:AB_2149542), anti–CD107a-PE (BioLegend, catalog no. 328608, RRID:AB_1186040), anti–B7-H3-APC (BioLegend, catalog no. 351006, RRID:AB_2564404), anti–PD-1-Alexa Fluor 488 (BioLegend, catalog no. 329936, RRID:AB_2563594), anti–PD-1-Brilliant Violet 421 (BioLegend, catalog no. 329920, RRID:AB_10960742), anti–PD-L1-PE (BioLegend, catalog no. 329706, RRID:AB_940368), anti–PD-L1-FITC (BioLegend, catalog no. 374510, RRID:AB_2734438), carboxyfluorescein diacetate succinimidyl ester (CFSE)/7-aminoactinomycin D (72782S, Cell Signaling Technology), anti–CD69-PE (BioLegend, catalog no. 310906, RRID:AB_314841), anti–NKG2D-APC (BioLegend, catalog no. 320808, RRID:AB_492962), anti–NKG2D-BV421 (BD Biosciences, catalog no. 568106, RRID:AB_2916841), anti– NKG2A-PE-Cy7 (BioLegend, catalog no. 375113, RRID:AB_2888865), anti-CD158-FITC (BioLegend, catalog no. 339504,RRID:AB_2130378), anti–CD159c-BV421 (BD Biosciences, catalog no. 748169, RRID: AB_2872630), anti–HA-Alexa Fluor 488 (BioLegend, catalog no. 901509, RRID:AB_2565072), anti–CD45-Brilliant Violet 510 (BioLegend, catalog no. 103138, RRID:AB_2563061), anti–CD45-APC-Cy7 (BioLegend, catalog no. 304014, RRID:AB_314402), B7-H3 protein (catalog no.: 11188-H08H-B, SinoBiological), anti–CD112-PE (BioLegend, catalog no. 337409, RRID:AB_2174163), anti–CD155-PE (BioLegend, catalog no. 337610, RRID:AB_2174019), and anti–HLA-E-PE (BioLegend, catalog no. 342604, RRID:AB_1659249). .. Antibodies specific for the following proteins were used for multiplex immunofluorescence staining: CD3 (Abcam, catalog no. ab16669, RRID: AB_443425, 1:300), granzyme B (Abcam, catalog no. ab4059, RRID: AB_304251, 1;1,000), CD56 (Cell Signaling Technology, catalog no. 3576, RRID: AB_2149540, 1:50), pan-CK (ab215838, 1:400), and CD8a (Cell Signaling Technology, catalog no. 70306, RRID: AB_2799781, 1:150).

    Control:

    Article Title: B7-H3 specific CAR-T cells exhibit potent activity against prostate cancer.
    Article Snippet: .. Cell Death Discovery (2023) 9:147 Cell Death Discovery (2023) 9:147 control T cells or CAR-T cells were incubated with the B7-H3 protein (SinoBiological, 11188-H02H) and then stained with IgG-Fc, followed by anti-CD3 antibody. ..

    Article Title: B7-H3 specific CAR-T cells exhibit potent activity against prostate cancer
    Article Snippet: .. For the analysis of CAR expression, control T cells or CAR-T cells were incubated with B7-H3 protein (SinoBiological, 11188-H02H), then stained with IgG-Fc, followed by anti-CD3 antibody. ..

    Incubation:

    Article Title: B7-H3 specific CAR-T cells exhibit potent activity against prostate cancer.
    Article Snippet: .. Cell Death Discovery (2023) 9:147 Cell Death Discovery (2023) 9:147 control T cells or CAR-T cells were incubated with the B7-H3 protein (SinoBiological, 11188-H02H) and then stained with IgG-Fc, followed by anti-CD3 antibody. ..

    Article Title: B7-H3 specific CAR-T cells exhibit potent activity against prostate cancer
    Article Snippet: .. For the analysis of CAR expression, control T cells or CAR-T cells were incubated with B7-H3 protein (SinoBiological, 11188-H02H), then stained with IgG-Fc, followed by anti-CD3 antibody. ..

    Staining:

    Article Title: B7-H3 specific CAR-T cells exhibit potent activity against prostate cancer.
    Article Snippet: .. Cell Death Discovery (2023) 9:147 Cell Death Discovery (2023) 9:147 control T cells or CAR-T cells were incubated with the B7-H3 protein (SinoBiological, 11188-H02H) and then stained with IgG-Fc, followed by anti-CD3 antibody. ..

    Article Title: B7-H3 specific CAR-T cells exhibit potent activity against prostate cancer
    Article Snippet: .. For the analysis of CAR expression, control T cells or CAR-T cells were incubated with B7-H3 protein (SinoBiological, 11188-H02H), then stained with IgG-Fc, followed by anti-CD3 antibody. ..

    Article Title: B7-H3-targeted natural killer cells effectively kill atypical teratoid / rhabdoid tumors and extend survival in orthotopic xenografts
    Article Snippet: B7-H3 surface staining on AT/RT cell lines was performed using anti-human B7-H3-APC (BioLegend, MIH42 clone). .. CAR expression analysis was performed by primary staining of NK cells with His-tagged recombinant 4Ig B7-H3 protein (Sino Biological 11188-H08H) followed by secondary staining with anti-His-PE or anti-His-APC (BioLegend). ..

    Expressing:

    Article Title: B7-H3 specific CAR-T cells exhibit potent activity against prostate cancer
    Article Snippet: .. For the analysis of CAR expression, control T cells or CAR-T cells were incubated with B7-H3 protein (SinoBiological, 11188-H02H), then stained with IgG-Fc, followed by anti-CD3 antibody. ..

    Article Title: B7-H3-targeted natural killer cells effectively kill atypical teratoid / rhabdoid tumors and extend survival in orthotopic xenografts
    Article Snippet: B7-H3 surface staining on AT/RT cell lines was performed using anti-human B7-H3-APC (BioLegend, MIH42 clone). .. CAR expression analysis was performed by primary staining of NK cells with His-tagged recombinant 4Ig B7-H3 protein (Sino Biological 11188-H08H) followed by secondary staining with anti-His-PE or anti-His-APC (BioLegend). ..

    Negative Control:

    Article Title: Anti-B7-H3 antibody and use thereof
    Article Snippet: .. Specifically, to activate a human T cell, the anti-CD3 antibody (UCHT1, Biolegend) was prepared by diluting it in PBS to be 5 μg/ml, and the B7-H3 protein (Sino Biological, 11188-H08H) or a negative control group, IgG1 (BioXCell, BP0297, labeled as IgG1 or Cont. ..

    Labeling:

    Article Title: Anti-B7-H3 antibody and use thereof
    Article Snippet: .. Specifically, to activate a human T cell, the anti-CD3 antibody (UCHT1, Biolegend) was prepared by diluting it in PBS to be 5 μg/ml, and the B7-H3 protein (Sino Biological, 11188-H08H) or a negative control group, IgG1 (BioXCell, BP0297, labeled as IgG1 or Cont. ..

    Recombinant:

    Article Title: B7-H3-targeted natural killer cells effectively kill atypical teratoid / rhabdoid tumors and extend survival in orthotopic xenografts
    Article Snippet: B7-H3 surface staining on AT/RT cell lines was performed using anti-human B7-H3-APC (BioLegend, MIH42 clone). .. CAR expression analysis was performed by primary staining of NK cells with His-tagged recombinant 4Ig B7-H3 protein (Sino Biological 11188-H08H) followed by secondary staining with anti-His-PE or anti-His-APC (BioLegend). ..



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